ABSTRACT
OBJECTIVE@#To investigate the expression and significance of miRNA-324-3p and its target gene WNT2B in tissue specimens of nasopharyngeal carcinoma (NPC) specimens.@*METHOD@#qRT-PCR was used to detect the expression of miRNA-324-3p and WNT2B mRNA, and Western blot was applied to assay the expression of WNT2B protein in 39 cases of NPC specimens and 21 cases of non-carcinoma epithelium. The relationship between their expression levels and clinicopathological characteristics and their correlation with clinical pathological parameters was analyzed.@*RESULT@#The expression of miRNA-324-3p was significantly down-regulated decreased but WNT2B mRNA/protein increased obviously in NPC specimens (P < 0.01). A negative correlation between miRNA-324-3p and WNT2B was spotted (P < 0.05). The expression levels of these markers were closely correlated with T stage, clinic stage and cervical lymph node metastasis (P < 0.05).@*CONCLUSION@#The loss of miRNA-324-3p and ectopic WNT2B might co-induce the initiation and progression of NPC.
Subject(s)
Humans , Carcinoma , Glycoproteins , Genetics , Metabolism , Lymphatic Metastasis , MicroRNAs , Metabolism , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Metabolism , Neoplasm Proteins , Metabolism , RNA, Messenger , Metabolism , Wnt Proteins , Genetics , MetabolismABSTRACT
Effects of total saponins from Prunella vulgaris (PVS)on acute myocardial infarction and hypertension of anesthetized rats were assessed. Pretreatment with PVS at a dose of 40mg/kg ip lowered Lg PVC, Lg (VT+VF) and ischemic arrhythmic scores; at a dose of 20mg/kg ip,resulted in a significant reduction of infarct size as compared with the controls; 2. 5mg/kg iv showed an hypotensive action to both systolic and diastolic blood pressure.
ABSTRACT
Herbalogical study shows that Xiakucao used in ancient times came from Prunella vulgaris and P. asiotica. The Portion used for medical purpose in ancient times was its stem and leaf, collected during the period of blooming. The portion used today was the spike, collected approach withering. Modern analytical determination showed that the aerial parts of three Prunella specics can also be used as medicine when collected in june and july.
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The pollen grains of 24 cultivarieties of Primus mimic of Rosaceae, which were collected from Shanghai, Hangzhou and Changxing. were observed by light microscope and scanning electron microscope. The pollen morphology of P.mume was first described as polytypism which could be distinguish into two classes and five types. Not only the pollen grains with 3-colporate. which were the primary form, but also the pollen grains with 4-colporate existed in many cultivarieties of P- tnume. The constitute of pollen types and sterility rate in each cultivarieties were also analysed. The result provides scientific reference for pollen identification, breeding and palynology.
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The isolated vessel elements from the xylem in Chinese Sarcandra species were observed by scanning electron microscope and optical microscope. The vessel elements with simple pits or (and) bor dered pits or (and) scalariform pits or (and) circular-scalariform pits in their secondary walls are 1200-1900 in length and all have scalariform multiperforation plates in which the numbers of bars are between 60 to 160.
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Objective:To study the high-performance liquid chromatographic(HPLC) fingerprints of different germplasms of Flos Carthami. Methods: HPLC was applied in chromatographic separation of 36 different germplasms of Flos Carthami collected from 11 provinces of China.The chromatography condition was: Agilent Zorbax C_(18) column(4.6 mm?250 mm,5 mm);mobile phase: CAN,0.5% H_3PO_4(from 1090 to 8020);running time: 60 min;flow speed: 1.0 ml/min;detection wavelength: 265 nm;and temperature: 20℃.The data were processed by Chromatographic Fingerprint Station designed by Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University.Different germplasms of Flos Carthami were subjected to cluster analysis and their similarity was also evaluated.Results: The different germplasms of Flos Carthami in this study were grouped into 2 chemical types.Samples from northwest of China were of high similarity;samples from the middle China were of high similarity to those from South China.The similarity of some germplasms of Flos Carthami was significantly different.Conclusion: The chemical components of different germplasms of Flos Carthami are obviously different from each other;selection of germplasm is very important in quality control of Flos Carthami.